Difference between revisions of "Part:BBa K1124009"

Latest revision as of 20:13, 27 September 2013

anti-tyrR sRNA

Usage and Biology

The Effects on tyrosine metabolism of TyrR

TyrR is a transcriptional regulator which represses the expression of several enzymes involved in the tyrosine producing pathway in E. coli. It was shown that TyrR repression leaded to increase of the tyrosine production.

RNA Silencing Utilizing MicC sRNA Scaffold

The combination of the target-binding sequence of a gene to be repressed with MicC sRNA scaffold(Part:BBa_K1124005) works as a tool of RNA silencing (see descriptions in MicC sRNA scaffold(Part:BBa_K1124005)). In this part,anti-tyrR sRNA, the target-binding sequence is complementary to the first 24bp of tyrR CDS(that is, the sequence from start codon ATG and the following 7 triplet codons). We expect that this part will repress tyrR expression in post-transcriptional way and therefore will increase tyrosine production. With this part, we constructed tyrosine synthesis device(Part:BBa_K1124106), which can likely increase tyrosine production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 '''RNA Silencing Utilizing MicC sRNA Scaffold'''
-The combination of the target-binding sequence of a gene to be repressed with MicC sRNA scaffold([[BBa_K1124005]]) works as a tool of RNA silencing (see descriptions in MicC sRNA scaffold([[BBa_K1124005]])). In this part,anti-tyrR sRNA, the target-binding sequence is complementary to the first 24bp of tyrR CDS(that is, the sequence from start codon ATG and the following 7 triplet codons).
+The combination of the target-binding sequence of a gene to be repressed with MicC sRNA scaffold([[Part:BBa_K1124005]]) works as a tool of RNA silencing (see descriptions in MicC sRNA scaffold([[Part:BBa_K1124005]])). In this part,anti-tyrR sRNA, the target-binding sequence is complementary to the first 24bp of tyrR CDS(that is, the sequence from start codon ATG and the following 7 triplet codons).
 We expect that this part will repress ''tyrR'' expression in post-transcriptional way and therefore will increase tyrosine production.
-With this part, we constructed tyrosine synthesis device([[BBa_K1124106]]), which can likely increase tyrosine production.
+With this part, we constructed tyrosine synthesis device([[Part:BBa_K1124106]]), which can likely increase tyrosine production.
 <span class='h3bb'>Sequence and Features</span>