Difference between revisions of "Part:BBa K1106004:Experience"

(User Reviews)
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As shown in the graphics below GFP production increases with the arsenite concentration.
 
As shown in the graphics below GFP production increases with the arsenite concentration.
  
'''Conclusion'''
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https://static.igem.org/mediawiki/2013/9/96/Gfpinduccion.jpg
The promoter is sensitive to different arsenite concentrations.
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[[File:Gfpinduccion.jpg ]]
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https://static.igem.org/mediawiki/2013/e/e3/Gfpampliacion2.jpg
  
[[File:Gfpampliacion2.jpg ]]
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'''Conclusion'''
 +
The promoter is sensitive to different arsenite concentrations.

Revision as of 20:03, 27 September 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1106004

User Reviews

UNIQ03f8343abcd79c53-partinfo-00000000-QINU UNIQ03f8343abcd79c53-partinfo-00000001-QINU

Objective Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concrentrations.

Methods Several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm).

Results As shown in the graphics below GFP production increases with the arsenite concentration.

Gfpinduccion.jpg

Gfpampliacion2.jpg

Conclusion The promoter is sensitive to different arsenite concentrations.