Difference between revisions of "Part:BBa K1106004:Experience"

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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===Applications of BBa_K1106004===
 
===Applications of BBa_K1106004===
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===User Reviews===
 
===User Reviews===
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As observed in the figure the GFP fluorescence increases with the arsenic concentration. This demonstrates that the rate of expression of any gene upregulated by this promoter is proportional to the arsenic concentration.
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'''Objective'''
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Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concrentrations.
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'''Methods'''
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Several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm).
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'''Results'''
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As shown in the graphics below GFP production increases with the arsenite concentration.
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'''Conclusion'''
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The promoter is sensitive to different arsenite concentrations.
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[[File:Gfpinduccion.jpg ]]
  
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[[File:Pars gfp.JPG]]
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[[File:Gfpampliacion2.jpg ]]

Revision as of 19:59, 27 September 2013

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Please enter how you used this part and how it worked out.

Applications of BBa_K1106004

User Reviews

UNIQ1f3bda9630def71a-partinfo-00000000-QINU UNIQ1f3bda9630def71a-partinfo-00000001-QINU

Objective Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concrentrations.

Methods Several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm).

Results As shown in the graphics below GFP production increases with the arsenite concentration.

Conclusion The promoter is sensitive to different arsenite concentrations.

File:Gfpinduccion.jpg

File:Gfpampliacion2.jpg