Difference between revisions of "Part:BBa K1084122:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1084122 short</partinfo>
 
<partinfo>BBa_K1084122 short</partinfo>
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===Design Notes===
 
===Design Notes===
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none
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This part is constructed to measure translation efficiency of SD4 (BBa_K1084102).
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We used LacZ&alpha; (BBa_I732006) as reporter gene and performed &beta;-Galactosidase assay.
  
  
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===Source===
 
===Source===
  
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none
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Based on Vimberg et al. (2007), we construnted this part from synthetic oligos.
  
 
===References===
 
===References===
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V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology

Latest revision as of 17:33, 27 September 2013

pTet SD4 LacZα dT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is constructed to measure translation efficiency of SD4 (BBa_K1084102). We used LacZα (BBa_I732006) as reporter gene and performed β-Galactosidase assay.


Source

Based on Vimberg et al. (2007), we construnted this part from synthetic oligos.

References

V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology