Difference between revisions of "Part:BBa K1074006:Experience"

 
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<partinfo>BBa_K1074006 AddReview 3</partinfo>
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<I>USTC_China iGEM13</I>
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In our project, we construct other four gene circuits based on this part. In these gene circuits, GFP is replaced by HBsAg, Ag85b, PAD4, LTB respectively. We transformed the recombinant
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Plasmids both in BL21 and Bacillus Subtilis WB800N and induced expression with IPTG . Results analyzed by SDS-page , mass spectrum and ELISA were proved positive. But proteins expressed in WB800N were far less than those in BL21. The antigenicity and transdermal function of N-terminal TD1 modified antigen HBsAg was also tested positive by ELISA.
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Latest revision as of 17:23, 27 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1074006

User Reviews

UNIQ076d8ad5e73f2796-partinfo-00000000-QINU UNIQ076d8ad5e73f2796-partinfo-00000001-QINU

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USTC_China iGEM13

In our project, we construct other four gene circuits based on this part. In these gene circuits, GFP is replaced by HBsAg, Ag85b, PAD4, LTB respectively. We transformed the recombinant Plasmids both in BL21 and Bacillus Subtilis WB800N and induced expression with IPTG . Results analyzed by SDS-page , mass spectrum and ELISA were proved positive. But proteins expressed in WB800N were far less than those in BL21. The antigenicity and transdermal function of N-terminal TD1 modified antigen HBsAg was also tested positive by ELISA.


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