Difference between revisions of "Part:BBa K1024003:Design"
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===Source=== | ===Source=== | ||
| − | <i>S. cerevisiae</i> | + | 1.<i>S. cerevisiae</i>: ADE2 |
| + | <br>2. Registry: K079046, K1119006 | ||
| + | <br>3. Dai' Lab, Tsinghua University: CYC1 Terminator | ||
===References=== | ===References=== | ||
Bellí G, Garí E, Piedrafita L, et al. An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast[J]. Nucleic acids research, 1998, 26(4): 942-947. | Bellí G, Garí E, Piedrafita L, et al. An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast[J]. Nucleic acids research, 1998, 26(4): 942-947. | ||
Latest revision as of 17:04, 27 September 2013
Reporter for Tet system in Yeast
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3077
Illegal BamHI site found at 2480
Illegal XhoI site found at 1779 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Design: The part is designed to valid the Tet system in yeast. TetR gene with VP16 is constitutively expressed, activating the downstream reporter gene of TetO and CYC1 TATA region. In the yeast Saccharomyces cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment. Thus, it could be used as a marker for screening of target phenotype.
Source
1.S. cerevisiae: ADE2
2. Registry: K079046, K1119006
3. Dai' Lab, Tsinghua University: CYC1 Terminator
References
Bellí G, Garí E, Piedrafita L, et al. An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast[J]. Nucleic acids research, 1998, 26(4): 942-947.