Difference between revisions of "Part:BBa K1024002:Design"

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===Design Notes===
 
===Design Notes===
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We constructed and improved standard BioBrick Parts about quorum sensing systems and modified the systems in prokaryotic microorganisms for usage in S. cerevisiae (Yeast). The LuxR gene is constitutively expressed, while the activation of Lux Promoter requires the signaling of N-Acyl Homoserine Lactone (AHL). Therefore, mCherry is activated in the presence of AHL.
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<p>We constructed and improved standard BioBrick Parts about quorum sensing systems and modified the systems in prokaryotic microorganisms for usage in S. cerevisiae (Yeast). The LuxR gene is constitutively expressed, while the activation of Lux Promoter requires the signaling of N-Acyl Homoserine Lactone (AHL). Therefore, mCherry is activated in the presence of AHL.</p>
  
 
===Source===
 
===Source===
  
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Yeast
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1.<i>S. cerevisiae</i>: pTEF
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<br>2.Registry: C0062, R0062
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<br>3.Dai's Lab, Tsinghua University: mCherry
  
 
===References===
 
===References===
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<br>[1]Fuqua W C, Winans S C, Greenberg E P. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators[J]. Journal of bacteriology, 1994, 176(2): 269.
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<br>[2]Sadowski I, Ma J, Triezenberg S, et al. GAL4-VP16 is an unusually potent transcriptional activator[J]. Nature, 1988, 335(6190): 563-564.

Latest revision as of 16:55, 27 September 2013

Reporter for quorum sensing systems in yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1580
    Illegal BamHI site found at 2546
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 506
    Illegal BsaI site found at 893
    Illegal BsaI site found at 1280
    Illegal BsaI.rc site found at 547
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 1321


Design Notes

We constructed and improved standard BioBrick Parts about quorum sensing systems and modified the systems in prokaryotic microorganisms for usage in S. cerevisiae (Yeast). The LuxR gene is constitutively expressed, while the activation of Lux Promoter requires the signaling of N-Acyl Homoserine Lactone (AHL). Therefore, mCherry is activated in the presence of AHL.

Source

1.S. cerevisiae: pTEF
2.Registry: C0062, R0062
3.Dai's Lab, Tsinghua University: mCherry

References


[1]Fuqua W C, Winans S C, Greenberg E P. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators[J]. Journal of bacteriology, 1994, 176(2): 269.
[2]Sadowski I, Ma J, Triezenberg S, et al. GAL4-VP16 is an unusually potent transcriptional activator[J]. Nature, 1988, 335(6190): 563-564.