Difference between revisions of "Part:BBa K1051303"

 
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<partinfo>BBa_K1051303 short</partinfo>
 
<partinfo>BBa_K1051303 short</partinfo>
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<h3>Purpose</h3>
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It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the sequence which is actived by clb6 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the  metabolic product of the Bacterial you use for transformation .
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Works to activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p.
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<br />
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<h3>Principle</h3>
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"clb6" is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the sequence which is actived by clb6 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the  metabolic product of the Bacterial you use for transformation .it most abundant during late G1; CLB6 has a paralog, CLB5, that arose from the whole genome duplication
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:54, 27 September 2013

clb6 promoter (during G1 phase)

Purpose

Works to activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p.

Principle

"clb6" is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the sequence which is actived by clb6 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation .it most abundant during late G1; CLB6 has a paralog, CLB5, that arose from the whole genome duplication

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]