Difference between revisions of "Part:BBa K1024004:Design"
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===References=== | ===References=== | ||
| + | <br>[1]Fuqua W C, Winans S C, Greenberg E P. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators[J]. Journal of bacteriology, 1994, 176(2): 269. | ||
| + | <br>[2]G, Garí E, Piedrafita L, et al. An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast[J]. Nucleic acids research, 1998, 26(4): 942-947. | ||
Latest revision as of 16:17, 27 September 2013
Sensor yeast (inducible TetR+VP16)
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2555
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1580
Illegal BamHI site found at 2546 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2555
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2555
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 506
Illegal BsaI site found at 893
Illegal BsaI site found at 1280
Illegal BsaI.rc site found at 547
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 1321
Design Notes
Design: The part was the sensor of the pathogen detector. LuxR was constitutively expressed. When sensing AHL, LuxR will bind to the Lux promoter and thus activated the downstream TetR with VP16. Mated with reporter yeast (BBa_K1024005), the TetR with VP16 will bind to the Tet operator and activated the downstream gene.
Source
S. cerevisiae & Registry
References
[1]Fuqua W C, Winans S C, Greenberg E P. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators[J]. Journal of bacteriology, 1994, 176(2): 269.
[2]G, Garí E, Piedrafita L, et al. An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast[J]. Nucleic acids research, 1998, 26(4): 942-947.