Difference between revisions of "Part:BBa K1149050:Design"

 
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===Design Notes===
 
===Design Notes===
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removed secretion tag to make intracellular
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We removed secretion tag to make intracellular. While doing this, we linearised the plasmid with PCR to exclude the secretion tag in construct BBa_K1149013. Then, we did blunt ended ligation to circularize the plasmid again. During the ligation, 3 nucleatodes disappeared fron the scar between the RBS and the start of the bdh2 gene. Therefore the scar is "TAC" istead of the regular "tactag".
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===Source===
 
===Source===
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<p>team: Imperial College 2013 iGEM Team </p>
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<p>organism: Ralstonia pickettii</p>
  
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Imperial iGEM 2013
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===References===
  
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===References===
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<b>Characterization of Two 3-Hydroxybutyrate Dehydrogenases in Poly(3-Hydroxybutyrate)-Degradable Bacterium,
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Ralstonia pickettii T1 </b>, Masahiko Takanashi and Terumi Saito, 2006

Latest revision as of 13:38, 27 September 2013


Bdh2 (intracellular)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 181
    Illegal AgeI site found at 1126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We removed secretion tag to make intracellular. While doing this, we linearised the plasmid with PCR to exclude the secretion tag in construct BBa_K1149013. Then, we did blunt ended ligation to circularize the plasmid again. During the ligation, 3 nucleatodes disappeared fron the scar between the RBS and the start of the bdh2 gene. Therefore the scar is "TAC" istead of the regular "tactag".

Source

team: Imperial College 2013 iGEM Team

organism: Ralstonia pickettii

References

Characterization of Two 3-Hydroxybutyrate Dehydrogenases in Poly(3-Hydroxybutyrate)-Degradable Bacterium, Ralstonia pickettii T1 , Masahiko Takanashi and Terumi Saito, 2006