Difference between revisions of "Part:BBa K1149050:Design"
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===References=== | ===References=== | ||
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| + | <b>Characterization of Two 3-Hydroxybutyrate Dehydrogenases in Poly(3-Hydroxybutyrate)-Degradable Bacterium, | ||
| + | Ralstonia pickettii T1 </b>, Masahiko Takanashi and Terumi Saito, 2006 | ||
Revision as of 13:36, 27 September 2013
Bdh2 (intracellular)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 181
Illegal AgeI site found at 1126 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We removed secretion tag to make intracellular. While doing this, we linearised the plasmid with PCR to exclude the secretion tag in construct BBa_K1149013. Then, we did blunt ended ligation to circularize the plasmid again. During the ligation, 3 nucleatodes disappeared fron the scar between the RBS and the start of the bdh2 gene. Therefore the scar is "TAC" istead of the regular "tactag".
Source
Imperial iGEM 2013
References
Characterization of Two 3-Hydroxybutyrate Dehydrogenases in Poly(3-Hydroxybutyrate)-Degradable Bacterium, Ralstonia pickettii T1 , Masahiko Takanashi and Terumi Saito, 2006