Difference between revisions of "Part:BBa K1149050"
Margarita K (Talk | contribs) |
Margarita K (Talk | contribs) |
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This part expresses Bdh2 3HB dehydrogenase enzyme intracellularly. | This part expresses Bdh2 3HB dehydrogenase enzyme intracellularly. | ||
+ | There is a GFP in operon with the enzyme which can be used to monitor expression levels. The promoter is arabinose inducible pBAD. | ||
http://www.igem.org/wiki/images/f/f0/Reaction_bdh.jpg | http://www.igem.org/wiki/images/f/f0/Reaction_bdh.jpg |
Revision as of 13:25, 27 September 2013
Bdh2 (intracellular)
This part expresses Bdh2 3HB dehydrogenase enzyme intracellularly. There is a GFP in operon with the enzyme which can be used to monitor expression levels. The promoter is arabinose inducible pBAD.
http://www.igem.org/wiki/images/f/f0/Reaction_bdh.jpg
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 181
Illegal AgeI site found at 1126 - 1000COMPATIBLE WITH RFC[1000]