Difference between revisions of "Part:BBa K1157013:Experience"

Latest revision as of 13:12, 27 September 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1157013

We used ELISA plate reader to read the fluorescence of this inhibitory circuit biosensor. Because fluorescence protein is constitutively expressed, this function tests the "reduced amount" of fluorescence. We culture the biosensor in LB overnight and dilute the medium until OD=0.1. We then add AHL into the medium to test results from 10-3 M to 10-9 M. Data is taken every hour from 0 to 6 hours. We can see that under higher AHL concentrations, the fluorescence is inhibited compared to the control group.

User Reviews

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 ===Applications of BBa_K1157013===
-We tested this circuit by transforming it in ''E. Coli'' DH5 alfa. We cultured the ''E. Coli'' in LB medium overnight, dilute until OD value=0.1, then added into ELISA plate reader for testing.(See testing protocol in NTU_Taida igem WIKI for details.) This is our functional results of testing BBa_K1157013 for 4 hours. [[File:pC_pRhl_mCherry.jpg]]
+We used ELISA plate reader to read the fluorescence of this inhibitory circuit biosensor. Because fluorescence protein is constitutively expressed, this function tests the "reduced amount" of fluorescence. We culture the biosensor in LB overnight and dilute the medium until OD=0.1. We then add AHL into the medium to test results from 10-3 M to 10-9 M. Data is taken every hour from 0 to 6 hours. We can see that under higher AHL concentrations, the fluorescence is inhibited compared to the control group.[[File:pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI.jpg]]
 ===User Reviews===