Difference between revisions of "Part:BBa K575024:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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| + | This functional testing is performed by 2013 igem team NTU_Taida. We used clinical bacterial species to test the expression of fluorescence in bacteria strains with different antibiotic resistance. These are the bacterial strains that we used. | ||
| + | (1) ''Acicetobacter baumannii'' ATCC baa-747 | ||
| + | (2) ''Pseudomonas aeruginosa'' PAO 1 | ||
| + | (3) ''Pseudomonas aeruginosa'' 1009373 | ||
| + | (4) ''Pseudomonas aeruginosa'' 2000671 | ||
| + | (5) ''Pseudomonas aeruginosa'' 0000765-1 | ||
| + | (6) ''Pseudomonas aeruginosa'' 0000963 | ||
| + | (7) ''Pseudomonas aeruginosa'' 0001596 | ||
| + | (8) ''Pseudomonas aeruginosa'' 0001819 | ||
| + | (9) ''Acicetobacter baumannii'' 8070903 | ||
| + | (10) ''Acicetobacter baumannii'' 0008115 | ||
| + | (11) ''Escherichia coli'' 0000424 | ||
| + | (12) ''Escherichia coli'' 0000703 | ||
| + | We use ELISA plate reader to read the fluorescence of these strains. BBa_K575024 were transformed into E. Coli DH5α and cultured overnight. It is then diluted until OD=0.1. Clinical species are also cultured overnight and centrifuged to get the supernatant, which acts as our testing target. We then add BBa_K575024 with the supernatant and react for 4 hours. Then we read the fluorescence intesity of these bacterial strains. | ||
| + | [[File:clinical species.jpg]] | ||
Latest revision as of 12:58, 27 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K575024
The Northwestern iGEM team constructed this part as a biosensor to detect the presence of Pseudomonas Aeuruginosa.
User Reviews
UNIQ48641a88d95c948d-partinfo-00000000-QINU UNIQ48641a88d95c948d-partinfo-00000001-QINU This functional testing is performed by 2013 igem team NTU_Taida. We used clinical bacterial species to test the expression of fluorescence in bacteria strains with different antibiotic resistance. These are the bacterial strains that we used. (1) Acicetobacter baumannii ATCC baa-747 (2) Pseudomonas aeruginosa PAO 1 (3) Pseudomonas aeruginosa 1009373 (4) Pseudomonas aeruginosa 2000671 (5) Pseudomonas aeruginosa 0000765-1 (6) Pseudomonas aeruginosa 0000963 (7) Pseudomonas aeruginosa 0001596 (8) Pseudomonas aeruginosa 0001819 (9) Acicetobacter baumannii 8070903 (10) Acicetobacter baumannii 0008115 (11) Escherichia coli 0000424 (12) Escherichia coli 0000703
We use ELISA plate reader to read the fluorescence of these strains. BBa_K575024 were transformed into E. Coli DH5α and cultured overnight. It is then diluted until OD=0.1. Clinical species are also cultured overnight and centrifuged to get the supernatant, which acts as our testing target. We then add BBa_K575024 with the supernatant and react for 4 hours. Then we read the fluorescence intesity of these bacterial strains.
