Difference between revisions of "Part:BBa K1157013"
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<partinfo>BBa_K1157013 short</partinfo> | <partinfo>BBa_K1157013 short</partinfo> | ||
| − | This biosensor is constructed to sense C-4 AHL molecules through a method different from BBa_K1157012. This time when AHL molecules are sensed, it activates Rhl promoter, which initiates the production of CI. Therefore, CI inhibits pCI and the expression of reporter mCherry is stopped. When compared to control group, the results will be expected to express lower fluorescence level when there is existence of AHL molecules. | + | The construct of this circuit is supported by igem team NTU_Taiwan. This biosensor is constructed to sense C-4 AHL molecules through a method different from BBa_K1157012. This time when AHL molecules are sensed, it activates Rhl promoter, which initiates the production of CI. Therefore, CI inhibits pCI and the expression of reporter mCherry is stopped. When compared to control group, the results will be expected to express lower fluorescence level when there is existence of AHL molecules. |
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Latest revision as of 11:43, 27 September 2013
pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI
The construct of this circuit is supported by igem team NTU_Taiwan. This biosensor is constructed to sense C-4 AHL molecules through a method different from BBa_K1157012. This time when AHL molecules are sensed, it activates Rhl promoter, which initiates the production of CI. Therefore, CI inhibits pCI and the expression of reporter mCherry is stopped. When compared to control group, the results will be expected to express lower fluorescence level when there is existence of AHL molecules.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 944
Illegal NheI site found at 967 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1241
Illegal BamHI site found at 2197 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1716
Illegal BsaI site found at 2672