Difference between revisions of "Part:BBa K1139201:Design"
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===Source===
 
===Source===
  
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The <i>phoA</i> promoter region of <i>E. coli</i> was amplified from MG1655 genomic DNA by PCR using upstream primer (5’-acgtgaattcgcggccgcttctagagaaagttaatcttttcaacagctgtcataaag-3’) and downstream primer (5’-ccgctactagtaaatacattaaaaaataaaaacaaagcgactataagtctc-3’). [http://regulondb.ccg.unam.mx/operon?term=ECK120014865&format=jsp&organism=ECK12&type=operon#phoAp <i>details</i>]
+
<i>phoA</i> promoter region of <i>E. coli</i> was amplified from MG1655 genomic DNA by PCR.<br>
 +
Upstream primer (5’-acgtgaattcgcggccgcttctagagaaagttaatcttttcaacagctgtcataaag-3’)<br>
 +
Downstream primer (5’-ccgctactagtaaatacattaaaaaataaaaacaaagcgactataagtctc-3’)<br> [http://regulondb.ccg.unam.mx/operon?term=ECK120014865&format=jsp&organism=ECK12&type=operon#phoAp <i>details</i>]
  
 
===References===
 
===References===

Revision as of 11:34, 27 September 2013

PphoA-GFP-TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 754


Design Notes

sequence confirmed

Source

phoA promoter region of E. coli was amplified from MG1655 genomic DNA by PCR.
Upstream primer (5’-acgtgaattcgcggccgcttctagagaaagttaatcttttcaacagctgtcataaag-3’)
Downstream primer (5’-ccgctactagtaaatacattaaaaaataaaaacaaagcgactataagtctc-3’)
[http://regulondb.ccg.unam.mx/operon?term=ECK120014865&format=jsp&organism=ECK12&type=operon#phoAp details]

References

1. M. Dollard et al. “Whole-cell bacterial sensors for the monitoring of phosphate availability” Journal of Microbiological Methods (2003)
2. H. Shinagawa et al. “Regulation of the pho Regulon in Escherichia coli K-12” J. Mol. Biol. (1983)
3. Y. Hsieh et al. “Global regulation by the seven-component Pi signaling system” Current Opinion in Microbiology (2010)
4. F. Neidhardt et al. “Culture Medium for Enterobacteria” Journal of Bacteriology (1974)
5. CCG/UNAM. Regulon DB. http://regulondb.ccg.unam.mx/index.jsp (accessed 2013-09-25)
6. D. R. Hoagland and D. I. Arnon (1950), The water-culture method for growing plants without soil. California agrigultual experiment station circular, 347, 1-32