Difference between revisions of "Part:BBa K1228006"
(→‎Experiment and Results)
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== Experiment and Results ==
 
== Experiment and Results ==
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1.Inoculate recombinant strains into 20ml LB.Cultivate at 37°C overnight.
+
1. Inoculate recombinant strains into 20ml LB.Cultivate at 37°C overnight.
 +
2. Add 200ul indicator inoculum (''Micrococcus Lysodeikticus'' or ''Escherichia Coli'') untill heated the LB solid medium cool to about 50°C.
 +
3.
 +
 
 
== Reference ==
 
== Reference ==
 
1.BellamyW,TakaseM,Yamauchi K,et al. Identification of the bactericidal domainof lactoferrin[J]. BiochimicaetBiophysicaActa,1992,1121( 1/2):130-136.
 
1.BellamyW,TakaseM,Yamauchi K,et al. Identification of the bactericidal domainof lactoferrin[J]. BiochimicaetBiophysicaActa,1992,1121( 1/2):130-136.
 
2.EliassenLT,Berge G,SveinbjornssonB,et al. Evidenceforadirect antitumormechanismof actionof Bovinel actoferricin[J].AnticancerResearch,2002,22(5):2703－2710.
 
2.EliassenLT,Berge G,SveinbjornssonB,et al. Evidenceforadirect antitumormechanismof actionof Bovinel actoferricin[J].AnticancerResearch,2002,22(5):2703－2710.
 
3.Wakabayashi H,TakaseM,TomitaM. Lactoferricin derived from milk protein lactoferrin[J]. Current Pharmaceutical Design,2003,9(16):1277－1287.
 
3.Wakabayashi H,TakaseM,TomitaM. Lactoferricin derived from milk protein lactoferrin[J]. Current Pharmaceutical Design,2003,9(16):1277－1287.

Revision as of 10:53, 27 September 2013

25aa with T1 terminator and Constitutive promoter veg

This generator contains BBa_K541503(https://parts.igem.org/Part:BBa_K541503) and BBa_K1228005(https://parts.igem.org/Part:BBa_K1228005). The generator can secrete 25 aminoacid peptide that is a part of Lactoferrin in B.subtilis. It has a broad anti-bacterial range and strong anti-bacterial activity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experiment and Results

1. Inoculate recombinant strains into 20ml LB.Cultivate at 37°C overnight. 2. Add 200ul indicator inoculum (Micrococcus Lysodeikticus or Escherichia Coli) untill heated the LB solid medium cool to about 50°C. 3.

Reference

1.BellamyW,TakaseM,Yamauchi K,et al. Identification of the bactericidal domainof lactoferrin[J]. BiochimicaetBiophysicaActa,1992,1121( 1/2):130-136. 2.EliassenLT,Berge G,SveinbjornssonB,et al. Evidenceforadirect antitumormechanismof actionof Bovinel actoferricin[J].AnticancerResearch,2002,22(5):2703－2710. 3.Wakabayashi H,TakaseM,TomitaM. Lactoferricin derived from milk protein lactoferrin[J]. Current Pharmaceutical Design,2003,9(16):1277－1287.