Difference between revisions of "Part:BBa K934025:Experience"

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===Applications of BBa_K934025===
 
===Applications of BBa_K934025===
  
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[[Image:Pluxtet_assay.png|thumb|center|500px|]]
 
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To characterize the function of the Lux/Tet hybrid promoter, we constructed a part Plux/tet-GFP (BBa_ K934024) by inserting Plux/tet promoter in front of a GFP coding sequence. By using the reporter cell that contains Plux/tet-GFP and constitutive LuxR and TetR generator (PlacIq-LuxR-TetR), we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, 3OC6HSL and aTc (anhydrous tetracycline).
 
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In the presence of both inducers, the culture showed about 500-fold higher fluorescence intensity than that in the absence of both inducers.
 
  
 
===User Reviews===
 
===User Reviews===
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<partinfo>BBa_K934025 AddReview 1</partinfo>
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<I>Tokyo-Tech iGEM 2013</I>
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HQ reported that the sequence of this part is inconsistent, but we couldn't find any error in our part as far as we saw the HQ's sequence result. It seems that the alignment result is wrong.
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Latest revision as of 09:40, 27 September 2013

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Please enter how you used this part and how it worked out.

Applications of BBa_K934025

User Reviews

UNIQ88775211d9da5e46-partinfo-00000000-QINU UNIQ88775211d9da5e46-partinfo-00000001-QINU

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Tokyo-Tech iGEM 2013

HQ reported that the sequence of this part is inconsistent, but we couldn't find any error in our part as far as we saw the HQ's sequence result. It seems that the alignment result is wrong.

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