Difference between revisions of "Part:BBa K1189024"
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This part was primarily used to construct <partinfo>BBa_K1189020</partinfo>.
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This part was primarily used to construct <partinfo>BBa_K1189020</partinfo> in order to allow us to express and purify the protein.
 
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Revision as of 07:53, 27 September 2013

Light chain human ferritin

This light ferritin chain comes from humans. This part along with heavy ferritin (BBa_K1189025), form the ferritin nanoparticle, an iron-storage particle made up of 24 subunits. The formed nanoparticle is highly robust, remaining stable at extreme pHs and temperatures.

This nanoparticle can also be used as a reporter when the iron core is modified with potassium ferrocyanide to form Prussian Blue. The Prussian Blue ferritin can then act as a peroxidase mimic, similar to horseradish peroxidase, resulting in colour changes in the presence of hydrogen peroxide, and TMB or ABTS.

This part was primarily used to construct BBa_K1189020 in order to allow us to express and purify the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 391