Difference between revisions of "Part:BBa K1139018"

Latest revision as of 07:49, 27 September 2013

Promoterless-M13 + Plac on pSB3

M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and lac promoter. The promoter upstream of g2p is deleted. The capsid is formed only when g2p is expressed.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 100
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 6026
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1139018 short</partinfo>
-M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell. This biobrick is derived from M13mp18 phage vector, amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and Lac promoter. The promoter on the upstream of g2(gene 2) is deleted.The capsid is formed only when g2p(gene 2 protein) is expressed.
+M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br>
+This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and <i>lac</i> promoter. The promoter upstream of <i>g2p</i> is deleted. The capsid is formed only when <i>g2p</i> is expressed.<br>
 <!-- Add more about the biology of this part here