Difference between revisions of "Part:BBa K1139018"
Tryalnigro (Talk | contribs) |
|||
| Line 3: | Line 3: | ||
M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br> | M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br> | ||
| − | This part is derived from M13mp18 phage vector, amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and <i>lac</i> promoter. The promoter on the upstream of <i>g2p</i> is deleted. The capsid is formed only when <i>g2p</i> is expressed.<br> | + | This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and <i>lac</i> promoter. The promoter on the upstream of <i>g2p</i> is deleted. The capsid is formed only when <i>g2p</i> is expressed.<br> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 07:48, 27 September 2013
Promoterless-M13 + Plac on pSB3
M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and lac promoter. The promoter on the upstream of g2p is deleted. The capsid is formed only when g2p is expressed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 100
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 6026
- 1000COMPATIBLE WITH RFC[1000]