Difference between revisions of "Part:BBa K1073009"
 
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<partinfo>BBa_K1073009 short</partinfo>
 
<partinfo>BBa_K1073009 short</partinfo>
  
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This construct is derived from <partinfo>BBa_C0078</partinfo> and <partinfo>BBa_B0015</partinfo>. It combines the autoinducer synthase LasI (producing N-3-oxo-dodecanoyl-HSL)including the LVA degradation tag with a double terminator to prevent further transciption.
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This construct is derived from <partinfo>BBa_C0078</partinfo> and <partinfo>BBa_B0015</partinfo>. It combines the autoinducer synthase LasI (producing N-3-oxo-dodecanoyl-HSL) including the LVA degradation tag with a double terminator to prevent further transciption.
  
  
 
===Usage and Biology===
 
===Usage and Biology===
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The synthetase LasI produces the autoinducer molecule N-3-oxo-dodecanoyl-HSL of the Las quorum sensing system.  
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The synthetase LasI produces the autoinducer molecule N-3-oxo-dodecanoyl-HSL of the las quorum sensing system.  
  
 
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Latest revision as of 07:33, 27 September 2013

LasI synthetase + LVA with subsequent double terminator

This construct is derived from BBa_C0078 and BBa_B0015. It combines the autoinducer synthase LasI (producing N-3-oxo-dodecanoyl-HSL) including the LVA degradation tag with a double terminator to prevent further transciption.


Usage and Biology

The synthetase LasI produces the autoinducer molecule N-3-oxo-dodecanoyl-HSL of the las quorum sensing system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 661
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]