Difference between revisions of "Part:BBa K1036006:Design"

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===References===
 
===References===
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1. <a href="http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP">http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP</a><br/>
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2. <a href="http://www.ncbi.nlm.nih.gov/pubmed/16369541">Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)</a><br/>
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3. <a href="http://www.ncbi.nlm.nih.gov/pubmed?term=A%20sensing%20array%20of%20radically%20coupled%20genetic%20'biopixels'.[all]&cmd=correctspelling">Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)</a>
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Revision as of 07:31, 27 September 2013


lux pL controlled luxR with lux pR controlled sfgfp (with LVA-tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3903
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 2080
    Illegal BsaI.rc site found at 3183
    Illegal SapI.rc site found at 1197


Design Notes

none


Source

The template of sfgfp gene namely biobrick pSB1C3-RBS-sfgfp-TT was provided by 2013 iGEM team Peking.We add the LVA-tag in the end of sfgfp.
We thanks for Peking's help.

References

1. http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP
2. Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)
3. Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)