Difference between revisions of "Part:BBa K1189024"
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<partinfo>BBa_K1189024 short</partinfo>
 
<partinfo>BBa_K1189024 short</partinfo>
  
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Light ferritin, along with heavy ferritin (hFTN INCLUDE LINKNKN), forms ferritin, a ubiquitous iron-storage nanoparticle. The formed nanoparticle is highly robust, remaining stable at extreme conditions, such as pHs of ______, temperatures of ____, and urea concentrations of _____.
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Light ferritin, along with heavy ferritin (<partinfo> BBa_K1189025</partinfo>), form the ferritin nanoparticle, the iron-storage protein for humans. The formed nanoparticle is highly robust, remaining stable at extreme conditions, such as pHs of ______, temperatures of ____, and urea concentrations of _____.
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The formed nanoparticle can also be used as a reporter when the iron core is modified with potassium ferrocyanide to form Prussian Blue. The Prussian Blue ferritin can then act as a peroxidase mimic, similar to horseradish peroxidase, resultinging in colour changes in the presence of hydrogen peroxide, and TMB or ABTS.
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This protein can serve two functions, it can serve as a molecular scaffold, a base upon which proteins can be anchored to.  
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When apart of fusions, ferritin has previously been found to stabilize proteins such as GFP, enhancing the effects of the fused protein.
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something scaffodl
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<includ link for ferritin>
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Ferritin has also been used as a reporter system, in a manner similar to horseradish peroxidase. This is done through a reaction known as the Fenton reaction, wherein hydrogen peroxide is homolytically cleaved to produce hydroxide radicals, which then react with certain substrates, such as TMB and ABTS, to produce a colourimetric change.
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<pictures or something>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:27, 27 September 2013

Light chain human ferritin

Light ferritin, along with heavy ferritin (No part name specified with partinfo tag.), form the ferritin nanoparticle, the iron-storage protein for humans. The formed nanoparticle is highly robust, remaining stable at extreme conditions, such as pHs of ______, temperatures of ____, and urea concentrations of _____. The formed nanoparticle can also be used as a reporter when the iron core is modified with potassium ferrocyanide to form Prussian Blue. The Prussian Blue ferritin can then act as a peroxidase mimic, similar to horseradish peroxidase, resultinging in colour changes in the presence of hydrogen peroxide, and TMB or ABTS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 391