Difference between revisions of "Part:BBa K1189018"
Line 4: Line 4:
 
<p>This part was created by fusing the heavy chain and light chains (PARtx2) of human ferritin together. It is expressed under the lacI promoter (PARt) and has a his-tag for protein purification. An E-coil (PARt) is included in order to allow binding of parts containing the respective K-coil (PART). </p>
 
<p>This part was created by fusing the heavy chain and light chains (PARtx2) of human ferritin together. It is expressed under the lacI promoter (PARt) and has a his-tag for protein purification. An E-coil (PARt) is included in order to allow binding of parts containing the respective K-coil (PART). </p>
 
<p>This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.</p>
 
<p>This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.</p>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2013/c/c7/UCalgary2013TRPrussianblueferritinsynthesis.png" alt="Prussian Blue Synthesis" width="800" height="401">
 +
<figcaption>
 +
<p><b>Figure 4.</b> Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period. </p>
 +
</figcaption>
 +
</figure>
 +
<br>
 +
<p> The resulting molecuel can then catalyse the formation of radicals from hydrogen perxoide, which can then cause a colour change in substrates such as TMB or ABTS.
 
<br>
 
<br>
 
<img src="https://static.igem.org/mediawiki/2013/a/a9/UCalgary2013TRSubstratecolour.png" alt="Substrate Colours" width="250" height="300">
 
<img src="https://static.igem.org/mediawiki/2013/a/a9/UCalgary2013TRSubstratecolour.png" alt="Substrate Colours" width="250" height="300">
 
<figcaption>
 
<figcaption>
 
<p><b>Figure 11.</b> Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.</p>
 
<p><b>Figure 11.</b> Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.</p>
 +
</figcaption>
 
<br>
 
<br>
 
Something about scaffold…  
 
Something about scaffold…  

Revision as of 07:22, 27 September 2013

Human ferritin di-subunit with E coil w/ LacI promoter

This part was created by fusing the heavy chain and light chains (PARtx2) of human ferritin together. It is expressed under the lacI promoter (PARt) and has a his-tag for protein purification. An E-coil (PARt) is included in order to allow binding of parts containing the respective K-coil (PART).

This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.


<img src="UCalgary2013TRPrussianblueferritinsynthesis.png" alt="Prussian Blue Synthesis" width="800" height="401"> <figcaption>

Figure 4. Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period.

</figcaption> </figure>

The resulting molecuel can then catalyse the formation of radicals from hydrogen perxoide, which can then cause a colour change in substrates such as TMB or ABTS.
<img src="UCalgary2013TRSubstratecolour.png" alt="Substrate Colours" width="250" height="300"> <figcaption> <p>Figure 11. Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.

</figcaption>
Something about scaffold…


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1289