Difference between revisions of "Part:BBa K091117:Experience"

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Because this las promoter is not characterized, we can't judge whether this las promoter is working or not. Therefore we improved this part. LasI promoter([https://parts.igem.org/Part:BBa_K649000 BBa_K649000]) which we constructed was successfully regulated by 3OC12-HSL.
 
Because this las promoter is not characterized, we can't judge whether this las promoter is working or not. Therefore we improved this part. LasI promoter([https://parts.igem.org/Part:BBa_K649000 BBa_K649000]) which we constructed was successfully regulated by 3OC12-HSL.
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<partinfo>BBa_K091117 AddReview 4</partinfo>
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<I>iGEM Team Braunschweig 2013</I>
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The part <partinfo>BBa_K091117</partinfo> was ordered from the iGEM registry and assembled with the RBS <partinfo>BBa_B0032</partinfo>.
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Using this device in combination with an ampicillin resistance gene (<partinfo>BBa_K1073003</partinfo>) cells showed a higher growth rate in ampicillin containing media when the promoter was induced. The device shows leakiness to a constant low degree in yeast extract complex media. Background activity of the genes downstream of the promoter was detected in all experiments. However activity was much higher when the promoter was induced by LasR and N-3-oxododecanoyl-HSL.
 
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Latest revision as of 07:18, 27 September 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K091117

User Reviews

UNIQ1cb62289ace6a992-partinfo-00000000-QINU UNIQ1cb62289ace6a992-partinfo-00000001-QINU

No review score entered. Tokyo-tech iGEM 2011

Because this las promoter is not characterized, we can't judge whether this las promoter is working or not. Therefore we improved this part. LasI promoter(BBa_K649000) which we constructed was successfully regulated by 3OC12-HSL.

;

UNIQ1cb62289ace6a992-partinfo-00000003-QINU

••••

iGEM Team Braunschweig 2013

The part BBa_K091117 was ordered from the iGEM registry and assembled with the RBS BBa_B0032. Using this device in combination with an ampicillin resistance gene (BBa_K1073003) cells showed a higher growth rate in ampicillin containing media when the promoter was induced. The device shows leakiness to a constant low degree in yeast extract complex media. Background activity of the genes downstream of the promoter was detected in all experiments. However activity was much higher when the promoter was induced by LasR and N-3-oxododecanoyl-HSL.

;