Difference between revisions of "Part:BBa K1189018"
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<p>This part was created by fusing the heavy chain and light chains (PARtx2) of human ferritin together. It is expressed under the lacI promoter (PARt) and has a his-tag for protein purification. An E-coil (PARt) is included in order to allow binding of parts containing the respective K-coil (PART). </p> | <p>This part was created by fusing the heavy chain and light chains (PARtx2) of human ferritin together. It is expressed under the lacI promoter (PARt) and has a his-tag for protein purification. An E-coil (PARt) is included in order to allow binding of parts containing the respective K-coil (PART). </p> | ||
<p>This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.</p> | <p>This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.</p> | ||
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<img src="https://static.igem.org/mediawiki/2013/a/a9/UCalgary2013TRSubstratecolour.png" alt="Substrate Colours" width="250" height="300"> | <img src="https://static.igem.org/mediawiki/2013/a/a9/UCalgary2013TRSubstratecolour.png" alt="Substrate Colours" width="250" height="300"> | ||
<figcaption> | <figcaption> | ||
<p><b>Figure 11.</b> Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.</p> | <p><b>Figure 11.</b> Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.</p> | ||
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Revision as of 07:13, 27 September 2013
Human ferritin di-subunit with E coil w/ LacI promoter
This part was created by fusing the heavy chain and light chains (PARtx2) of human ferritin together. It is expressed under the lacI promoter (PARt) and has a his-tag for protein purification. An E-coil (PARt) is included in order to allow binding of parts containing the respective K-coil (PART).
This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.
<img src="" alt="Substrate Colours" width="250" height="300">
<figcaption>
Figure 11. Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.
Something about scaffold…
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1289