Difference between revisions of "Part:BBa K1189018"
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<p>This part was created by fusing the heavy chain and light chains (PARtx2) of human ferritin together. It is expressed under the lacI promoter (PARt) and has a his-tag for protein purification. An E-coil (PARt) is included in order to allow binding of parts containing the respective K-coil (PART). </p>
 
<p>This part was created by fusing the heavy chain and light chains (PARtx2) of human ferritin together. It is expressed under the lacI promoter (PARt) and has a his-tag for protein purification. An E-coil (PARt) is included in order to allow binding of parts containing the respective K-coil (PART). </p>
 
<p>This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.</p>
 
<p>This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.</p>
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[[Image:UCalgary2013TRSubstratecolour.png|300px]]
 
<br>
 
<br>
 
<img src="https://static.igem.org/mediawiki/2013/a/a9/UCalgary2013TRSubstratecolour.png" alt="Substrate Colours" width="250" height="300">
 
<img src="https://static.igem.org/mediawiki/2013/a/a9/UCalgary2013TRSubstratecolour.png" alt="Substrate Colours" width="250" height="300">

Revision as of 07:06, 27 September 2013

Human ferritin di-subunit with E coil w/ LacI promoter

This part was created by fusing the heavy chain and light chains (PARtx2) of human ferritin together. It is expressed under the lacI promoter (PARt) and has a his-tag for protein purification. An E-coil (PARt) is included in order to allow binding of parts containing the respective K-coil (PART).

This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.

300px
<img src="UCalgary2013TRSubstratecolour.png" alt="Substrate Colours" width="250" height="300">


It would act as a peroxidase-mimic, utilizing H2O2 to oxidize substrates such as TMB and ABTS to produce a colourimetric output.


<figure> <img src="UCalgary2013TRSubstratecolour.png" alt="Substrate Colours" width="250" height="300"> <figcaption>

Figure 11. Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.

</figcaption> </figure> Something about scaffold…


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1289