Difference between revisions of "Part:BBa K1073003:Experience"
(→Applications of BBa_K1073003) |
(→User Reviews) |
||
| Line 16: | Line 16: | ||
<I>iGEM Team Braunschweig 2013</I> | <I>iGEM Team Braunschweig 2013</I> | ||
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
| − | Using this device in combination with an ampicillin resistance gene cells showed a higher growth rate in ampicillin containing media when the promoter was induced. The device shows leakiness to a constant low degree in yeast extract complex media. Background activity of the genes downstream of the promoter were detected in all experiments. However activity was much higher when the promoter was induced by LasR and N-oxododecanoyl-HSL. | + | Using this device in combination with an ampicillin resistance gene cells showed a higher growth rate in ampicillin containing media when the promoter was induced. The device shows leakiness to a constant low degree in yeast extract complex media. Background activity of the genes downstream of the promoter were detected in all experiments. However activity was much higher when the promoter was induced by LasR and N-3-oxododecanoyl-HSL. |
|}; | |}; | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_K1073003 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1073003 EndReviews</partinfo> | ||
Revision as of 07:06, 27 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1073003
The iGEM Team Braunschweig 2013 used this device in combination with an ampicillin resistance gene (BBa_K1073000) to induce growth by LasR and N-3-oxododecanoyl-HSL of different E.coli strains in ampicillin containing media.
User Reviews
UNIQ5c060301d8086420-partinfo-00000001-QINU
|
••••
iGEM Team Braunschweig 2013 |
Using this device in combination with an ampicillin resistance gene cells showed a higher growth rate in ampicillin containing media when the promoter was induced. The device shows leakiness to a constant low degree in yeast extract complex media. Background activity of the genes downstream of the promoter were detected in all experiments. However activity was much higher when the promoter was induced by LasR and N-3-oxododecanoyl-HSL. |
UNIQ5c060301d8086420-partinfo-00000003-QINU