Difference between revisions of "Part:BBa K1189018"

 
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<partinfo>BBa_K1189018 short</partinfo>
 
<partinfo>BBa_K1189018 short</partinfo>
  
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ASDASD
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<p>This part was created by fusing the heavy chain and light chains (PARtx2) of human ferritin together. It is expressed under the lacI promoter (PARt) and has a his-tag for protein purification. An E-coil (PARt) is included in order to allow binding of parts containing the respective K-coil (PART). </p>
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<p>This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.</p>
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<br>
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<figure>
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<img src="https://static.igem.org/mediawiki/2013/c/c7/UCalgary2013TRPrussianblueferritinsynthesis.png" alt="Prussian Blue Synthesis" width="800" height="401">
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<figcaption>
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<p><b>Figure 4.</b> Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period. </p>
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</figcaption>
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</figure>
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<br>
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<p>
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It would act as a peroxidase-mimic, utilizing H2O2 to oxidize substrates such as TMB and ABTS to produce a colourimetric output. </p>
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<br>
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<figure>
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<img src="https://static.igem.org/mediawiki/2013/a/a9/UCalgary2013TRSubstratecolour.png" alt="Substrate Colours" width="250" height="300">
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<figcaption>
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<p><b>Figure 11.</b> Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.</p>
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</figcaption>
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</figure>
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Something about scaffold…
  
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===Usage and Biology===
 
  
 
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Revision as of 07:00, 27 September 2013

Human ferritin di-subunit with E coil w/ LacI promoter

This part was created by fusing the heavy chain and light chains (PARtx2) of human ferritin together. It is expressed under the lacI promoter (PARt) and has a his-tag for protein purification. An E-coil (PARt) is included in order to allow binding of parts containing the respective K-coil (PART).

This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.


<figure> <img src="UCalgary2013TRPrussianblueferritinsynthesis.png" alt="Prussian Blue Synthesis" width="800" height="401"> <figcaption>

Figure 4. Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period.

</figcaption> </figure>

It would act as a peroxidase-mimic, utilizing H2O2 to oxidize substrates such as TMB and ABTS to produce a colourimetric output.


<figure> <img src="UCalgary2013TRSubstratecolour.png" alt="Substrate Colours" width="250" height="300"> <figcaption>

Figure 11. Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.

</figcaption> </figure> Something about scaffold…


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1289