Difference between revisions of "Part:BBa K1073000:Design"
 
(One intermediate revision by the same user not shown)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
The sequence for the beta-lactamase was gained by PCR of the original BBa_P1002 with appropiate primers. The primer sequences can be found on iGEM Team Braunschweig's wiki 2013.
+
The sequence for the beta-lactamase was gained by PCR of the original BBa_P1002 with appropiate primers excluding the RBS sequence. The primer sequences can be found on iGEM Team Braunschweig's wiki 2013.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
The part was derived from BBa_P1002 ampicillin resistance Cassette via PCR. For further Information about the source see BBa_P1002.
+
The part was derived from BBa_P1002 ampicillin resistance cassette via PCR. For further Information about the source see BBa_P1002.
  
  
 
===References===
 
===References===

Latest revision as of 06:59, 27 September 2013

ampicillin resistance


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence for the beta-lactamase was gained by PCR of the original BBa_P1002 with appropiate primers excluding the RBS sequence. The primer sequences can be found on iGEM Team Braunschweig's wiki 2013.

Source

The part was derived from BBa_P1002 ampicillin resistance cassette via PCR. For further Information about the source see BBa_P1002.


References