Difference between revisions of "Part:BBa K1139150"

Revision as of 04:21, 27 September 2013

Prm/lac-GFP-TT

Prm/lac is a hybrid promoter that is modified to be activated by lamda repressor (CI) and repressed by LacI repressor.
On the downstream of the promoter, GFP is inserted as a reporter.

Fig. 1-A. Our new designed hybrid promoter

To characterize the function of the rm/lac hybrid promoter (BBa_K1139151), we constructed this part Prm/lac-GFP (BBa_K1139150) by inserting rm/lac promoter in front of a GFP coding sequence. By using the reporter cell that contains Prm/lac-GFP, we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, CI and IPTG (Fig. 1-B).
We confirmed that our new rm/lac hybrid promoter was actually activated by CI through an induction assay (Fig. 1-C).

Fig. 1B. Fluorescence intensity detected by flow cytometer

Fig. 1C. Comparison of N99 and JM2.300

For more information, see [http://2013.igem.org/Team:Tokyo_Tech/Experiment/RM-lac_Hybrid_Promoter_Assay our work in Tokyo_Tech 2013 wiki].

Sequence and Features