Difference between revisions of "Part:BBa K1195001"

Revision as of 22:46, 26 September 2013

Amylase A

The fundamental component of bacterial biofilms is the extracellular polymeric substance, which is composed primarily of exopolysaccharides and proteins. The EPS forms the framework matrix for bacterial biofilms, and composes 50% to 90% of the total organic matter of the biofilm.

α-Amylase is a calcium metalloenzyme that hydrolyzes alpha bonds of long-chain carbohydrates. As a metalloenzyme, α-Amylase is nonfunctional in the absence of calcium. In the presence of calcium, α-Amylase is capable of inhibiting biofilm growth by hydrolyzing the polysaccharides that help compose the extracellular polymeric substance that is the fundamental component of bacterial biofilms.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 874
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 874
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 874
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 874
1000
COMPATIBLE WITH RFC[1000]

Characterization Data

@@ Line 11: / Line 11: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+===Sequence and Features===
 <partinfo>BBa_K1195001 SequenceAndFeatures</partinfo>
-atgCGTAATC CCACGCTGTT ACAATGTTTT CACTGGTATT ACCCGGAAGG CGGTAAGCTC
-TGGCCTGAAC TGGCCGAGCG CGCCGACGGT TTTAATGATA TTGGTATCAA TATGGTCTGG
-TTGCCGCCCG CCTATAAAGG CGCATCGGGC GGGTATTCGG TCGGCTACGA CTCCTATGAT
-TTATTTGATT TAGGCGAGTT TGATCAGAAA GGCAGCATCC CTACTAAATA TGGCGATAAA
-GCACAACTGC TGGCCGCCAT TGATGCTCTG AAACGTAATG ACATTGCGGT GCTGTTGGAT
-GTGGTAGTCA ACCACAAAAT GGGCGCGGAT GAAAAAGAAG CTATTCGCGT GCAGCGTGTA
-AATGCTGATG ACCGTACGCA AATTGACGAA GAAATCATTG AGTGTGAAGG CTGGACGCGT
-TACACCTTCC CCGCCCGTGC CGGGCAATAC TCGCAGTTTA TCTGGGATTT CAAATGTTTT
-AGCGGTATCG ACCATATCGA AAACCCTGAC GAAGATGGCA TTTTTAAAAT TGTTAACGAC
-TACACCGGCG AAGGCTGGAA CGATCAGGTT GATGATGAAT TAGGTAATTT CGATTATCTG
-ATGGGCGAGA ATATCGATTT TCGCAATCAT GCCGTGACGG AAGAGATTAA ATACTGGGCG
-CGCTGGGTGA TGGAACAAAC GCAATGCGAC GGTTTTCGTC TTGATGCGGT CAAACATATT
-CCAGCCTGGT TTTATAAAGA GTGGATCGAA CACGTACAGG AAGTTGCGCC AAAGCCGCTG
-TTTATTGTGG CGGAGTACTG GTCGCATGAA GTTGATAAGC TGCAAACGTA TATTGATCAG
-GTGGAAGGCA AAACCATGCT GTTTGATGCG CCGCTGCAGA TGAAATTCCA TGAAGCATCG
-CGCATGGGGC GCGACTACGA CATGACGCAG ATTTTCACGG GTACATTAGT GGAAGCCGAT
-CCTTTCCACG CCGTGACGCT CGTTGCCAAT CACGACACCC AACCGTTGCA AGCCCTCGAA
-GCGCCGGTCG AACCGTGGTT TAAACCGCTG GCGTATGCCT TAATTTTGTT GCGGGAAAAT
-GGCGTTCCTT CGGTATTCTA TCCGGACCTC TACGGTGCGC ATTACGAAGA TGTCGGTGGT
-GACGGGCAAA CCTATCCGAT AGATATGCCA ATAATCGAAC AGCTTGATGA GTTAATTCTC
-GCCCGTCAGC GTTTCGCCCA CGGTGTACAG ACGTTATTTT TCGACCATCC GAACTGCATT
-GCCTTTAGCC GCAGTGGCAC CGACGAATTT CCCGGCTGCG TGGTGGTCAT GTCGAACGGG
-GATGATGGCG AAAAAACCAT TCATCTGGGA GAGAATTACG GCAATAAAAC CTGGCGTGAT
-TTTCTGGGGA ACCGGCAAGA GAGAGTAGTG ACCGACGAAA ACGGCGAAGC AACCTTCTTT
-TGCAACGGCG GCAGCGTCAG CGTGTGGGTT ATCGAAGAGG TGATTtaa
 <!-- Uncomment this to enable Functional Parameter display
@@ Line 46: / Line 22: @@
 ===Characterization Data===
-[[File:BYU2013-BiofilmAssayGraph1.jpg]]
+<div style="text-align:center;">[[Image:BYU2013-BiofilmAssayGraph1.jpg]]</div>