Difference between revisions of "Part:BBa J100129:Experinece"

(Created page with "This promoter was not cloned successfully as indicated by the presence of just green fluorescence-- not the red fluorescence or cells without fluorescence. The following is a gra...")
 
 
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This promoter was not cloned successfully as indicated by the presence of just green fluorescence-- not the red fluorescence or cells without fluorescence. The following is a graph detailing the fluorescence per optical density. This is not a measure of our promoter.
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Our promoter J100129 was not cloned successfully as indicated by the presence of just green fluorescence. We tried to clone it into [[Part:BBa_J119137]], and it we had, the colonies would not be green. Unfortunately, all of ours were green.  The following is a graph detailing the fluorescence per optical density. This is not a measure of our promoter since we did not clone it into the plasmid. This is part of an undergraduate biology lab assignment. Our positive control is [[Part:BBa_J04450]] with a strong promoter.  
  
  
 
[[File:113SP.png]]
 
[[File:113SP.png]]

Latest revision as of 20:17, 26 September 2013

Our promoter J100129 was not cloned successfully as indicated by the presence of just green fluorescence. We tried to clone it into Part:BBa_J119137, and it we had, the colonies would not be green. Unfortunately, all of ours were green. The following is a graph detailing the fluorescence per optical density. This is not a measure of our promoter since we did not clone it into the plasmid. This is part of an undergraduate biology lab assignment. Our positive control is Part:BBa_J04450 with a strong promoter.


113SP.png