Difference between revisions of "Part:BBa K1017811"
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<partinfo>BBa_K1017811 short</partinfo> | <partinfo>BBa_K1017811 short</partinfo> | ||
| − | Pcons is a constitutive promoter which can be used to turn the expression level. rRBS-2 is a sRNA binding site that is designed by iGEM13_NCTU_Formosa. When the sRNA from K1017402 bind on it, this part will be shut down. Then, the downstream gene won't be made. mRFP(E1010) is a red fluorescent protein that functions as a reporter | + | Pcons is a constitutive promoter which can be used to turn the expression level. rRBS-2 is a sRNA binding site that is designed by iGEM13_NCTU_Formosa. When the sRNA from K1017402 bind on it, this part will be shut down. Then, the downstream gene mRFP won't be made. mRFP(E1010) is a red fluorescent protein that functions as a reporter. |
We made this biobrick in order to test whether our self-designed rRBS-2 really works. | We made this biobrick in order to test whether our self-designed rRBS-2 really works. | ||
| − | Also, we want to compare this rRBS-2’s strength with other RBS(B0030, B0032, B0034 | + | Also, we want to compare this rRBS-2’s strength with other RBS(B0030, B0032, B0034). |
| + | [[File:NCTU_Test_functional_test_of_different_RBS.png|center|600px|Figure 1. From left to right, the ribosome biding sites respectively: B0032, K1017202, B0030, B0034, control.]] | ||
| + | In the figure, we can see that B0034 is the strongest RBS, and our self-designed rRBS-2 is just behind it. B0030 and B0032 are at the third and the forth place. This result indicates that the rRBS-2 really works and performs well. | ||
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Latest revision as of 18:31, 26 September 2013
Pcons+rRBS-2+mRFP+J61048
Pcons is a constitutive promoter which can be used to turn the expression level. rRBS-2 is a sRNA binding site that is designed by iGEM13_NCTU_Formosa. When the sRNA from K1017402 bind on it, this part will be shut down. Then, the downstream gene mRFP won't be made. mRFP(E1010) is a red fluorescent protein that functions as a reporter. We made this biobrick in order to test whether our self-designed rRBS-2 really works. Also, we want to compare this rRBS-2’s strength with other RBS(B0030, B0032, B0034).
In the figure, we can see that B0034 is the strongest RBS, and our self-designed rRBS-2 is just behind it. B0030 and B0032 are at the third and the forth place. This result indicates that the rRBS-2 really works and performs well.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 784
Illegal AgeI site found at 625
Illegal AgeI site found at 737 - 1000COMPATIBLE WITH RFC[1000]