Difference between revisions of "Part:BBa K1017811"

Latest revision as of 18:31, 26 September 2013

Pcons+rRBS-2+mRFP+J61048

Pcons is a constitutive promoter which can be used to turn the expression level. rRBS-2 is a sRNA binding site that is designed by iGEM13_NCTU_Formosa. When the sRNA from K1017402 bind on it, this part will be shut down. Then, the downstream gene mRFP won't be made. mRFP(E1010) is a red fluorescent protein that functions as a reporter. We made this biobrick in order to test whether our self-designed rRBS-2 really works. Also, we want to compare this rRBS-2’s strength with other RBS(B0030, B0032, B0034).

Figure 1. From left to right, the ribosome biding sites respectively: B0032, K1017202, B0030, B0034, control.

In the figure, we can see that B0034 is the strongest RBS, and our self-designed rRBS-2 is just behind it. B0030 and B0032 are at the third and the forth place. This result indicates that the rRBS-2 really works and performs well.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 784
Illegal AgeI site found at 625
Illegal AgeI site found at 737
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1017811 short</partinfo>
-Pcons is a constitutive promoter family member(from J23100 to J23119) which can be used to tune the expression level of express part and we choose J23101. rRBS-2 is a sRNA binding site that designed by iGEM13_NCTU_Formosa. When the sRNA from BBa_K1017402 bind on it, this part will be shut down. mRFP(E1010) is a red fluorescent protein. J61048 is a terminator.
+Pcons is a constitutive promoter which can be used to turn the expression level. rRBS-2 is a sRNA binding site that is designed by iGEM13_NCTU_Formosa. When the sRNA from K1017402 bind on it, this part will be shut down. Then, the downstream gene mRFP won't be made. mRFP(E1010) is a red fluorescent protein that functions as a reporter.
+We made this biobrick in order to test whether our self-designed rRBS-2 really works.
+Also, we want to compare this rRBS-2’s strength with other RBS(B0030, B0032, B0034).
+[[File:NCTU_Test_functional_test_of_different_RBS.png|center|600px|Figure 1. From left to right, the ribosome biding sites respectively: B0032, K1017202, B0030, B0034, control.]]
+In the figure, we can see that B0034 is the strongest RBS, and our self-designed rRBS-2 is just behind it. B0030 and B0032 are at the third and the forth place. This result indicates that the rRBS-2 really works and performs well.
 <!-- Add more about the biology of this part here