Difference between revisions of "Part:BBa K1061003"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1061003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1061003 SequenceAndFeatures</partinfo>
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====Functional experiment====
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We test the death-induction effect of RIP 3 in two kinds of cell lines, here's the result.We also characterized it quantitively by using FACS.
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[[Image:Suicidegene-rip3-new.png|thumb|center|500px|functional experiment of RIP 3]]
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====kill range====
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For each suicide gene, it is important to know which cancer cells and normal cells it will kill.So that people can speficially choose gene that will be functional in their project when duelling about cancer. And we define this new feature for each suicide gene that we submitted. The list will be changed due to more and more experiment will be done. We highly recommend people to adopt this feature as an important characteristic of suicide gene.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 18:19, 26 September 2013

RIP 3

Receptor interacting protein 3, also a serine/threonine kinase that play an important role in cell apoptosis and necrosis. It can induce necrosis via the interaction with RIP 1.[1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 623
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 267
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1198

Functional experiment

We test the death-induction effect of RIP 3 in two kinds of cell lines, here's the result.We also characterized it quantitively by using FACS.

functional experiment of RIP 3

kill range

For each suicide gene, it is important to know which cancer cells and normal cells it will kill.So that people can speficially choose gene that will be functional in their project when duelling about cancer. And we define this new feature for each suicide gene that we submitted. The list will be changed due to more and more experiment will be done. We highly recommend people to adopt this feature as an important characteristic of suicide gene.

References:

[1] Duan-Wu Zhang et al. 2009,RIP3, an Energy Metabolism Regulator That Switches TNF-Induced Cell Death from Apoptosis to Necrosis,Science 325:332-336