Difference between revisions of "Part:BBa K1036001"
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Revision as of 17:31, 26 September 2013
the ndh gene coding for respiratory NADH dehydrogenase II
Description
ndh is the coding gene for NADH dehydrogenase II (NCBI), a non-proton-translating single subunit membrane bond enzyme important in maintaining a redox-balanced dinucleotide pool. NADH dehydrogenase II is the preponderant respiratory source of H2O2 as well as O2-. In fact, substantial H2O2 was formed when quinoneless vesicles containing only NADH dehydrogenase II were incubated with NADH.
Earlier studies estimated that the formation rate of intracellular O2- was about 10-5 M S-1 by extrapolation of O2- yield from this enzyme. And NADH dehydrogenase II formed about 4 O2- per 10 molecules of H2O2 both during respiration and in the quinoneless membranes.
Experimental Data
Construciton
SDS-PAGE
The result was showed in Fig.1 and ndh means without AHL and ndh+AHL means with AHL. The red and blue arrows showed the two up-regulation proteins, while the green arrow showed the down-regulation protein.According to the LC-MS-MS data, the band showed by the red arrow was the ndh gene.
![](https://static.igem.org/mediawiki/2013/c/c9/XMU-China_ndh.png)
Sequence and Features
Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1360
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]