Difference between revisions of "Part:BBa K1139150"
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<partinfo>BBa_K1139150 short</partinfo> | <partinfo>BBa_K1139150 short</partinfo> | ||
− | Prm/lac is a hybrid promoter that is modified to be activated by lamda repressor (CI) and repressed by LacI repressor. | + | Prm/lac is a hybrid promoter that is modified to be activated by lamda repressor (CI) and repressed by LacI repressor.<br> |
− | On the downstream of the promoter, | + | On the downstream of the promoter, GFP is inserted as a reporter.<br> |
− | [[Image:Titech2013_parts_K1139150_Fig1A.jpg|thumb|center|500px| | + | [[Image:Titech2013_parts_K1139150_Fig1A.jpg|thumb|center|500px|<b>Fig. 1-A.</b> Our new designed hybrid promoter]] |
− | To characterize the function of the | + | To characterize the function of the <i>rm/lac</i> hybrid promoter (<partinfo>BBa_K1139151</partinfo>), we constructed this part Prm/lac-GFP (<partinfo>BBa_K1139150</partinfo>) by inserting ''rm/lac'' promoter in front of a GFP coding sequence. By using the reporter cell that contains Prm/lac-GFP, we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, CI and IPTG (Fig. 1-B). <br> |
− | We confirmed that our new ''rm/lac'' hybrid promoter was actually activated by CI through an induction assay (Fig. 1-C). | + | We confirmed that our new ''rm/lac'' hybrid promoter was actually activated by CI through an induction assay (Fig. 1-C).<br> |
− | [[Image:Titech2013_parts_K1139150_Fig1B.jpg|thumb|center|500px| | + | [[Image:Titech2013_parts_K1139150_Fig1B.jpg|thumb|center|500px|<b>Fig. 1B.</b> Fluorescence intensity detected by flow cytometer]] |
− | [[Image:Titech2013_parts_K1139150_Fig1C.jpg|thumb|center|500px| | + | [[Image:Titech2013_parts_K1139150_Fig1C.jpg|thumb|center|500px|<b>Fig. 1C.</b> Comparison of N99 and JM2.300]] |
Revision as of 17:26, 26 September 2013
Prm/lac-GFP-TT
Prm/lac is a hybrid promoter that is modified to be activated by lamda repressor (CI) and repressed by LacI repressor.
On the downstream of the promoter, GFP is inserted as a reporter.
To characterize the function of the rm/lac hybrid promoter (BBa_K1139151), we constructed this part Prm/lac-GFP (BBa_K1139150) by inserting rm/lac promoter in front of a GFP coding sequence. By using the reporter cell that contains Prm/lac-GFP, we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, CI and IPTG (Fig. 1-B).
We confirmed that our new rm/lac hybrid promoter was actually activated by CI through an induction assay (Fig. 1-C).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 769