Difference between revisions of "Part:BBa K1036001:Design"
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===References=== | ===References=== | ||
| + | Messner, K. R. & Imlay, J. A. The identification of primary sites of superoxide and hydrogen peroxide formation in the aerobic respiratory chain and sulfite reductase complex of <i>Escherichia coli</i>. J. Biol. Chem. 274, 10119-10128 (1999) | ||
Revision as of 16:35, 26 September 2013
the ndh gene coding for respiratory NADH dehydrogenase II
Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1360
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
the LVA-tag was added
Source
the ndh gene was amplifed from the genome DNA of E.coli strain BL21 via PCR
References
Messner, K. R. & Imlay, J. A. The identification of primary sites of superoxide and hydrogen peroxide formation in the aerobic respiratory chain and sulfite reductase complex of Escherichia coli. J. Biol. Chem. 274, 10119-10128 (1999)