Difference between revisions of "Part:BBa K1036000:Design"
Tina Zhang (Talk | contribs) (→Design Notes) |
Tina Zhang (Talk | contribs) (→Source) |
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===Source=== | ===Source=== | ||
| − | The ''ndh'' gene was amplifed from the genome of ''E. coli'' strain BL21(DE3) via PCR. | + | The ''ndh'' gene was amplifed from the genome DNA of ''E. coli'' strain BL21(DE3) via PCR. |
===References=== | ===References=== | ||
1. Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012) | 1. Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012) | ||
Revision as of 15:49, 26 September 2013
lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2544
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101
Design Notes
The ndh gene was amplifed from the genome of E. coli strain BL21(DE3) via PCR. And we know that the biobrick BBa_F2621 (position in plate: 2012-Plate 2-21F) is a quorum sensing promoter. We digested ndh with EcoRI and Xba I, and 21F with EcoRI and SpeI. Then we constructed ndh plasmid by ligation.
Source
The ndh gene was amplifed from the genome DNA of E. coli strain BL21(DE3) via PCR.
References
1. Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)