Difference between revisions of "Part:BBa K1017726"

Revision as of 15:28, 26 September 2013

Pcons+B0030+pcya+B0032+ho1+B0030

We design this part to change heme into phycocyanobilin(PCB). The following is the pathway. The part of the photoreceptor that responds to light, phycocyanobilin, is not naturally produced in E. coli. We therefore introduced two phycocyanobilin-biosynthesis genes (ho1 and pcyA) from Synechocystis that convert heme into phycocyanobilin.

In this part, Pcons is a constitutive promoter family member(from J23100 to J23119) which can be used to tune the expression level of express part. And what we choose is J23101. B0030 and B0032 are strong ribosome binding sites(RBS), but they have different strength. pcyA and hol1 are two requisite genes which are required for the biosynthesis to change heme into PCB. hol1 will oxidizes the heme group then generate biliverdin IXalpha, and pcyA converts biliverdin IXalpha into phycocyanobilin(PCB).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 833
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 386
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 We design this part to change heme into phycocyanobilin(PCB). The following is the pathway.
-The part of the photoreceptor that responds to light, phycocyanobilin, is not naturally produced in E. coli. We therefore introduced two phycocyanobilin-biosynthesis genes (ho1 and pcyA) from Synechocystis that convert haem into phycocyanobilin.
+The part of the photoreceptor that responds to light, phycocyanobilin, is not naturally produced in E. coli. We therefore introduced two phycocyanobilin-biosynthesis genes (ho1 and pcyA) from Synechocystis that convert heme into phycocyanobilin.
 https://static.igem.org/mediawiki/2013/9/9b/Nctu_formosa_PCBsynthe.jpg