Difference between revisions of "Part:BBa K1119000:Design"
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pCMV myc/mito(Invitrogen™) | pCMV myc/mito(Invitrogen™) | ||
http://tools.lifetechnologies.com/content/sfs/manuals/pshooter_pcmv_man.pdf | http://tools.lifetechnologies.com/content/sfs/manuals/pshooter_pcmv_man.pdf | ||
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Dean, J., Tran , L., Liao, J., Dipple, K., Reue, K., Tontonoz, P., & Beaven, S. (2009). Resistance to diet-induced obesity in mice with synthetic glyoxylate shunt. Cell Metabolism, 9((6)), 525–536. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/19490907 | Dean, J., Tran , L., Liao, J., Dipple, K., Reue, K., Tontonoz, P., & Beaven, S. (2009). Resistance to diet-induced obesity in mice with synthetic glyoxylate shunt. Cell Metabolism, 9((6)), 525–536. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/19490907 | ||
Revision as of 15:01, 26 September 2013
Mitochondrial Leader Sequence in RFC10 standard
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is in RFC10 standard but cannot be fused directly to other protein domains due to limitations in RFC10. Users who obtained this part can obtain the part by PCR and fuse to other domains using Splicing by Overlapping PCR.
Source
The CDS of MLS was cloned out from pCMV/myc/mito (Invitrogen, Carlsbard, CA) using PCR with primers that includes prefix and suffix in RFC10 standard. The original source of this CDS of MLS sequence is from that of subunit VIII of human cytochrome c oxidase. (Invitrogen, Carlsbard, CA)
References
pCMV myc/mito(Invitrogen™)
http://tools.lifetechnologies.com/content/sfs/manuals/pshooter_pcmv_man.pdf
Dean, J., Tran , L., Liao, J., Dipple, K., Reue, K., Tontonoz, P., & Beaven, S. (2009). Resistance to diet-induced obesity in mice with synthetic glyoxylate shunt. Cell Metabolism, 9((6)), 525–536. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/19490907