Difference between revisions of "Part:BBa C0020:Design"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_C0020 short</partinfo>
 
<partinfo>BBa_C0020 short</partinfo>
  
 
<partinfo>BBa_C0020 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_C0020 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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A BioBricks restriction site for PstI was at position 260. As a result, the codon starting at position 262 was changed from "gca" to "gcg".
 
A BioBricks restriction site for PstI was at position 260. As a result, the codon starting at position 262 was changed from "gca" to "gcg".
  
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The terminating codon at the end, "tga", was changed to "taataa" as per BioBrick regulations as told to us by Randy Rettberg.
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The terminating codon at the end, "tga", was changed to "taataa" as per the Registry's [[Help:Protein coding#Regulations_for_Protein_Coding_Parts|BioBrick regulations for coding parts]].
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===Source===
 
===Source===
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*Organism: NCBI - Escherichia coli K12 MG1655 (NC_000913)
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*Paper: P Matsumura, J J Rydel, R Linzmeier and D Vacante.  Journal of Bacteriology. 1984 "Overexpression and sequence of the Escherichia coli cheY gene and biochemical activities of the CheY protein." [http://jb.asm.org/cgi/content/abstract/160/1/36?vol=160&fp=36&view=abstract link]
  
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NCBI - Escherichia coli K12 MG1655 (NC_000913)
 
  
 
===References===
 
===References===
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*Databases:
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* <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=946393">NCBI sequence data</a>
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** UniProt ID [http://www.pir.uniprot.org/cgi-bin/upEntry?id=CHEY_ECOLI P0AE67; P06143]
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** SwissProt data available [http://ca.expasy.org/cgi-bin/niceprot.pl?CHEY_ECOLI here]
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** NCBI sequence data [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=946393 946393]
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** EchoBase [http://www.biolws1.york.ac.uk/echobase/Gene.cfm?recordID=EB0148 EB0148]

Latest revision as of 15:19, 20 July 2006

CheY "tumbling" chemotaxis coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Changes from the NCBI sequence: A BioBricks restriction site for PstI was at position 260. As a result, the codon starting at position 262 was changed from "gca" to "gcg".

The terminating codon at the end, "tga", was changed to "taataa" as per the Registry's BioBrick regulations for coding parts.


Source

  • Organism: NCBI - Escherichia coli K12 MG1655 (NC_000913)
  • Paper: P Matsumura, J J Rydel, R Linzmeier and D Vacante. Journal of Bacteriology. 1984 "Overexpression and sequence of the Escherichia coli cheY gene and biochemical activities of the CheY protein." [http://jb.asm.org/cgi/content/abstract/160/1/36?vol=160&fp=36&view=abstract link]


References

  • Databases:
    • UniProt ID [http://www.pir.uniprot.org/cgi-bin/upEntry?id=CHEY_ECOLI P0AE67; P06143]
    • SwissProt data available [http://ca.expasy.org/cgi-bin/niceprot.pl?CHEY_ECOLI here]
    • NCBI sequence data [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=946393 946393]
    • EchoBase [http://www.biolws1.york.ac.uk/echobase/Gene.cfm?recordID=EB0148 EB0148]