Difference between revisions of "Part:BBa K1119009:Design"

Revision as of 14:45, 26 September 2013

CMV promoter - MLS - GFP - hGH polyA tail

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 614
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 628
Illegal AgeI site found at 1462
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1865
Illegal BsaI.rc site found at 1367

There are NgoMIV & AgeI sites around MLS CDS (BBa_K1119001) and GFP reporter CDS(BBa_K648013), which make the part incompatible with RFC25 standard.

HKUST iGEM team 2013

Revision as of 14:44, 26 September 2013 (view source) Klchuab (Talk \| contribs) ← Older edit		Revision as of 14:45, 26 September 2013 (view source) Klchuab (Talk \| contribs) Newer edit →
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	===Design Notes===		===Design Notes===
−	There are NgoMIV & AgeI sites around MLS CDS ([[Part:BBa_K1119001\|BBa_K1119001]]) and GFP reporter CDS([[Part:BBa_K648013\|BBa_K648013]], which make the part incompatible with RFC25 standard.	+	There are NgoMIV & AgeI sites around MLS CDS ([[Part:BBa_K1119001\|BBa_K1119001]]) and GFP reporter CDS([[Part:BBa_K648013\|BBa_K648013]]), which make the part incompatible with RFC25 standard.