Difference between revisions of "Part:BBa K1084102:Design"

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<partinfo>BBa_K1084102 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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This RBS has an sequence called an "enhancer".
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This RBS has an sequence called an "enhancer". The enhancer is located upstream of the SD seqeuence. It enhances the binding of Ribosome and mRNA.
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The enhancer is located upstream of the SD seqeuence. It enhances the binding of Ribosome and mRNA.
+
−
 
+
 
Also, it is said that the strength of the RBS, depends on the length of SD sequence.
 
Also, it is said that the strength of the RBS, depends on the length of SD sequence.
 +
 +
  
 
===Source===
 
===Source===
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Based on Vimberg et al. (2007), we construnted this part from synthetic oligos.
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coming soon...
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===References===
 
===References===
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V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology

Latest revision as of 14:06, 26 September 2013

SD4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This RBS has an sequence called an "enhancer". The enhancer is located upstream of the SD seqeuence. It enhances the binding of Ribosome and mRNA. Also, it is said that the strength of the RBS, depends on the length of SD sequence.


Source

Based on Vimberg et al. (2007), we construnted this part from synthetic oligos.

References

V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology