Difference between revisions of "Part:BBa K1084102:Design"
| (2 intermediate revisions by one other user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1084102 short</partinfo> | <partinfo>BBa_K1084102 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | This RBS has an sequence called an "enhancer". The enhancer is located upstream of the SD seqeuence. It enhances the binding of Ribosome and mRNA. | |
| + | Also, it is said that the strength of the RBS, depends on the length of SD sequence. | ||
===Source=== | ===Source=== | ||
| − | + | Based on Vimberg et al. (2007), we construnted this part from synthetic oligos. | |
| − | + | ||
===References=== | ===References=== | ||
| + | V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology | ||
Latest revision as of 14:06, 26 September 2013
SD4
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This RBS has an sequence called an "enhancer". The enhancer is located upstream of the SD seqeuence. It enhances the binding of Ribosome and mRNA. Also, it is said that the strength of the RBS, depends on the length of SD sequence.
Source
Based on Vimberg et al. (2007), we construnted this part from synthetic oligos.
References
V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology