Difference between revisions of "Part:BBa K1100061"

Revision as of 14:03, 26 September 2013

RNA-OUT S1 It is the wild type RNA-OUT of a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT. It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1100061 short</partinfo>
+It is the wild type RNA-OUT of a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT. It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%.
-coming soon
+[[File:Example.jpg]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===