Difference between revisions of "Part:BBa K737024:Experience"

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<I>Tokyo-Tech iGEM 2013</I>
 
<I>Tokyo-Tech iGEM 2013</I>
 
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<b>1. Sequence</b><br>
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No NotI restriction site in prefix and suffix. <br>
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1bp deletion and 2bp mutation exist between promoter and RBS. (They are not fatal to the function)<br>
  
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<b>2. Result of our experiment</b><br>
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We improved this OUC-China’s phosphate sensor part including <i>phoB</i> promoter by amplifying the <i>phoA</i> promoter region of <i>E. coli</i> (MG1655) and ligating it upstream of GFP part (<partinfo>BBa_K1139200</partinfo>)<br>
  
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No NotI restriction site in prefix and suffix.  
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[[Image:Titech2013_parts_K1139201_Fig4.jpg|thumb|left|500px|'''Fig. 1.''' Our <i>phoA</i> promoter assay result]]
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1bp deletion and 2bp mutation exist between promoter and RBS. (They are not fatal to the function)
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[[Image:Titech2013_parts_K1139201_Fig5.jpg|thumb|left|500px|'''Fig. 2.''' OUC-China 2012’s <i>phoB</i> promoter assay result (converted to bar chart)]]
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<br><br><br><br><br><br><br><br><br><br><br><br>
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Compared to OUC-China’s phosphate sensor part including <i>phoB</i> promoter (Fig. 2), our phosphate sensor part shows clearer result (Fig. 1) (<partinfo>BBa_K1139200</partinfo>, <partinfo>BBa_K1139201</partinfo>).

Revision as of 13:32, 26 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K737024

User Reviews

UNIQ75e1b80c6fe1e9ae-partinfo-00000000-QINU UNIQ75e1b80c6fe1e9ae-partinfo-00000001-QINU

•••••

Tokyo-Tech iGEM 2013

1. Sequence
No NotI restriction site in prefix and suffix.
1bp deletion and 2bp mutation exist between promoter and RBS. (They are not fatal to the function)

2. Result of our experiment
We improved this OUC-China’s phosphate sensor part including phoB promoter by amplifying the phoA promoter region of E. coli (MG1655) and ligating it upstream of GFP part (BBa_K1139200)

Fig. 1. Our phoA promoter assay result
Fig. 2. OUC-China 2012’s phoB promoter assay result (converted to bar chart)













Compared to OUC-China’s phosphate sensor part including phoB promoter (Fig. 2), our phosphate sensor part shows clearer result (Fig. 1) (BBa_K1139200, BBa_K1139201).