Difference between revisions of "Part:BBa K1150011"

Revision as of 12:44, 26 September 2013

SV40 promoter

SV40 promotor is a viral promotor derived from Simian virus 40.

Because we could not found the SV40 promotor in the parts registry, we decided to provide one to the following iGEM teams. This enables the teams to choose from differently strong mammalian promotors – dependent of the individual intention. We tested both promotors (BBa_K1150011 [SV40], BBa_K747096 [CMV]) with our own devices for expression rate and effectivity. For further information please take a look at BBa_K1150019 and BBa_K1150020.

In an earlier study CMV and SV40 promoter were tested in different B and T cell as well as in two non-lymphoid cell. In all cell lines the CMV promoter construct was higher expressed than that of SV40 [Zarrin et al., 1999].

In comparison to the cellular promoter VV1 SV40 showed similar activities. Hence with an SV40 promotor one can gain a more physiologic control for reporter assays compared to CMV.

ZARRIN, A.; MALKIN, L.; FONG, I.; LUK, K.; GHOSE, A.; BERINSTEIN, N. (1999). Comparison of CMV, RSV, SV40 viral and VV1 cellular promoters in B and T lymphoid and non-lymphoid cell lines. Biochimica et Biophysica Acta 1446: 135-139.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1150011 short</partinfo>
-SV40  promotor is a viral promotor derived from Simian virus 40.
+SV40 promotor is a viral promotor derived from Simian virus 40.
 Because we could not found the SV40 promotor in the parts registry, we decided to provide one to the following iGEM teams. This enables the teams to choose from differently strong mammalian promotors – dependent of the individual intention.
@@ Line 12: / Line 12: @@
-ZARRIN, A.; MALKIN, L.; FONG, I.; LUK, K.; GHOSE, A.; BERINSTEIN, N. (1999). Comparison of CMV, RSV, SV40 viral and VV1 cellular promoters in B and T lymphoid and non-lymphoid cell lines. Biochimica et Biophysica Acta 1446: 135-139.
+ZARRIN, A.; MALKIN, L.; FONG, I.; LUK, K.; GHOSE, A.; BERINSTEIN, N. (1999). Comparison of CMV, RSV, SV40 viral and VV1 cellular promoters in B and T lymphoid and non-lymphoid cell lines. ''Biochimica et Biophysica Acta'' 1446: 135-139.